A molecular mechanism for the repression of transcription by the H‐NS protein
- 1 December 2001
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 42 (5) , 1311-1323
- https://doi.org/10.1046/j.1365-2958.2001.02706.x
Abstract
The H-NS protein is a major component of the bacterial nucleoid and plays a crucial role in the global gene regulation of enteric bacteria. Although H-NS does not exhibit a high DNA sequence specificity, a number of H-NS-responsive promoters have been shown to contain regions of intrinsic DNA curvature located either upstream or downstream of the transcription start point. We have studied H-NS binding to DNA and in vitro transcriptional regulation by H-NS at several synthetic promoters with or without curved sequences inserted upstream of the Pribnow box. We show how such inserts determine the final organization of H-NS-containing nucleoprotein complexes and how this affects transcription. We refine a two-step mechanism for the constitution of H-NS assemblies that are efficient in regulation.Keywords
This publication has 59 references indexed in Scilit:
- The Downstream Regulatory Element of the proU Operon of Salmonella typhimurium Inhibits Open Complex Formation by RNA Polymerase at a DistanceJournal of Biological Chemistry, 2000
- Conformational changes of the upstream DNA mediated by H-NS and FIS regulate E. coli rrnB P1 promoter activity 1 1Edited by M. YanivJournal of Molecular Biology, 1999
- Lac and λ repressors relieve silencing of the Escherichia coli bgl promoter. activation by alteration of a repressing nucleoprotein complexJournal of Molecular Biology, 1998
- DNA Sequence-Specific Recognition by the Saccharomyces cerevisiae “TATA” Binding Protein: Promoter-Dependent Differences in the Thermodynamics and Kinetics of BindingBiochemistry, 1998
- Molecular aspects of the E. coli nucleoid protein, H-NS: a central controller of gene regulatory networksFEMS Microbiology Letters, 1997
- Fluorescence Analysis of the Escherichia coli Transcription Regulator H-NS Reveals Two Distinguishable Complexes Dependent on Binding to Specific or Nonspecific DNA SitesPublished by Elsevier ,1995
- Modulated expression of promoters containing upstream curved DNA sequences by the Escherichia coli nucleoid protein H‐NSMolecular Microbiology, 1994
- Interaction of the main cold shock protein CS7.4 (CspA) of Escherichia coli with the promoter region of hnsBiochimie, 1994
- Expression of the gene encoding the major bacterial nucleoid protein H‐NS is subject to transcriptional auto‐repressionMolecular Microbiology, 1993
- Sequence determinants for H1 binding on Escherichia coli lac and gal promotersBiochemistry, 1990