Establishment of a human cell line after transformation by a plasmid containing the early region of the SV40 genome
- 1 January 1983
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 7 (1) , 73-81
- https://doi.org/10.1016/0309-1651(83)90106-6
Abstract
A human clonal cell line, designated TAH9, was established from cells of adipose tissue by fusion with Escherichia coli protoplasts containing a recombinant plasmid carrying the early genes of SV40. The establishment of the cell line is preceded by a delayed growth crisis. Apparently after cell transformation, another event(s) of low frequency is required for cell immortalization. Some biological properties of TAH9 cells are also described.This publication has 11 references indexed in Scilit:
- Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells.Proceedings of the National Academy of Sciences, 1981
- Study of liver differentiation in vitro.The Journal of cell biology, 1981
- Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.Proceedings of the National Academy of Sciences, 1980
- Human fibroblasts transformed by the early region of SV40 DNA: Analysis of “free” viral DNA sequencesVirology, 1980
- Transformation of isolated rat hepatocytes with simian virus 40.The Journal of cell biology, 1980
- Establishment of a fetal rat liver cell line that retains differentiated liver functions.Proceedings of the National Academy of Sciences, 1980
- Direct transfer of cloned genes from bacteria to mammalian cells.Proceedings of the National Academy of Sciences, 1980
- Expression of simian virus 40 early genes in transformed rat cells is correlated with maintenance of the transformed phenotypeProceedings of the National Academy of Sciences, 1978
- Establishment of clonal human placental cells synthesizing human choriogonadotropin.Proceedings of the National Academy of Sciences, 1978
- SV40‐induced transformation of human diploid cells: Crisis and recoveryJournal of Cellular and Comparative Physiology, 1965