Selection of Plasma for Hepatitis B Immune Globulin Using a Low Cost Radioimmunoassay

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Abstract
Polystyrene beads coated with pasteurized polyethylene glycol-precipitated HBsAg [hepatitis B surface antigen] were used to detect anti-HBs by a solid phase inhibition assay employing unreacted 125I-anti-HBs previously processed by polyethylene glycol precipitation and used to test for HBsAg. A weak anti-HBs (approximately 0.1 IU/ml) positive control was used to establish a target level of reactivity. A total of 16,035 freshly donated units of [human] blood, a 90 sample coded anti-HBs panel and a selection of hyperimmune globulins were investigated. All positive results were confirmed by a standard commercial assay. Antibody content of selected samples was quantitated in international units. A target level of anti-HBs activity is suggested for donor plasma to be fractionated for the production of hepatitis B immune globulin.