An Improved Method for the Purification of Sweet Potato β-Amylase*
- 1 October 1963
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 54 (4) , 375-377
- https://doi.org/10.1093/oxfordjournals.jbchem.a127801
Abstract
The method consists of aqueous extraction of the acetone-dried powder, heating (60[degree]C, 10 minutes), precipitation with an equal volume of acetone, ammonium sulfate fractionation between 0.2 and 0.7 saturation, acid treatment (pH 3.6), and crystallization by adding saturated ammonium sulfate solution adjusted to pH 3.6. The re-crystallized preparation possessed about 30-fold specific activity, and was shown to be homogeneous by electrophoretic and ultra centrifugal analyses.Keywords
This publication has 0 references indexed in Scilit: