Evaluation of Phagocytic Function of Canine Peripheral Polymorphonuclear Leucocytes by Whole Blood Chemiluminescence.

1 January 1992

journal article
Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science

Vol. 54 (1) , 63-67
https://doi.org/10.1292/jvms.54.63

Abstract

Zymosan-induced and luminol-aided chemiluminescence (CL) of whole blood from beagle dogs was estimated for the function of polymorphonuclear leucocytes (PMNs). Whole blood (0.1 ml) was examined directly and results were obtained within 20 min. A phagocytic function of PMNs can be estimated from the peak CL counts and the number of PMNs in a specimen, and the opsonic activity can also be estimated by the peak time showing peak CL after the addition of non-opsonized zymosan. The optimal temperatures to keep diluted whole blood for the CL measurement was around 13 degrees C. Thus, this method offers information concerning the functions of phagocytic cells in whole blood.

Keywords

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