Cloning of Metalloprotease Genes in the Carpet Viper (Echis pyramidurm leakeyi)
Open Access
- 1 September 1994
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 224 (2) , 483-488
- https://doi.org/10.1111/j.1432-1033.1994.00483.x
Abstract
The genes coding for two metalloproteases, EcH‐I and EcH‐II, have been cloned from an Echis pyramidum leakeyi venom gland cDNA library. The cDNA sequences predict two zymogen molecules with strong amino acid sequence similarity and the same domain structure present in other members of the viper metalloprotease/disintegrin gene family. EcH‐I and EcH‐II contain pro‐protein, enzyme and disintegrin domains. Analysis of the cDNAs coding for EcH‐I, EcH‐II, jararhagin, trigramin and Ht‐e reveals a strong similarity, particularly in the untranslated regions and regions coding for the pro‐peptide. Comparison of EcH‐I and EcH‐II with venom metalloproteases, mammalian matrix‐degrading metalloproteases, sperm proteins, and a potential tumour suppressor gene highlights the presence of a number of motifs with potential functional significance.Keywords
This publication has 29 references indexed in Scilit:
- Genomic sequences encoding the acidic and basic subunits of Mojave toxin: unusually high sequence identity of non-coding regionsGene, 1994
- A novel metalloprotease/disintegrin–like gene at 17q21.3 is somatically rearranged in two primary breast cancersNature Genetics, 1993
- Structural and functional aspects of RGD-containing protein antagonists of glycoprotein IIb-IIIaCurrent Opinion in Biotechnology, 1993
- The matrix‐degrading metalloproteinasesBioEssays, 1992
- Amino acid sequence of fibrolase, a direct‐acting fibrinolytic enzyme from agkistrodon contortrix contortrix venomProtein Science, 1992
- A potential fusion peptide and an integrin ligand domain in a protein active in sperm–egg fusionNature, 1992
- A common precursor for a putative hemorrhagic protein and rhodostomin, a platelet aggregation inhibitor of the venom of Calloselasma rhodostoma: Molecular cloning and sequence analysisBiochemical and Biophysical Research Communications, 1991
- Basic Local Alignment Search ToolJournal of Molecular Biology, 1990
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Rapid and Sensitive Protein Similarity SearchesScience, 1985