DISSOCIATION BETWEEN HEMODYNAMIC AND PLATELET ANTI-AGGREGANT EFFECTS OF PROSTACYCLIN IN DOGS DETERMINED WITH A RAPID PLATELET ISOLATION TECHNIQUE

  • 1 January 1983
    • journal article
    • research article
    • Vol. 102  (1) , 133-139
Abstract
Prostacyclin, a potent vasodilator and inhibitor of platelet aggregation, is being increasingly used in disease states in which platelets may play a pathophysiologic role. A literature review revealed a variable dissociation between the in vitro platelet antiaggregant activity of prostacyclin and both the in vivo hemodynamic and platelet antiaggregant activities. Because widely variable platelet isolation techniques may have contributed to this variability, the hemodynamic and platelet antiaggregant effects of prostacyclin infusion were studied in the dog, using in vitro techniques limiting the time from blood sampling to aggregometry to 2.5 and 10.5 min. The short technique demonstrated complete inhibition of platelet aggregation at a prostacyclin infusion rate that caused only a mild decrease in arterial pressure. The longer technique required an 8-fold higher prostacyclin infusion rate to achieve the same degree of antiaggregant activity. The prostacyclin activity apparent half-life was 3 min. Because interspecies differences exist in both the hemodynamic and antiaggregant effects of prostacyclin and in their relationship to each other, a closer approximation of in vivo antiaggregant effects may be obtained with in vitro methods that are rapid, thus taking into account the lability of prostacyclin. The variable in vitro platelet antiaggregant effects of prostacyclin reported in the literature are due to the variable and longer techniques.