A C‐terminal proline is required for bioluminescence of the Ca2+‐binding photoprotein, aequorin
- 16 December 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 295 (1-3) , 63-66
- https://doi.org/10.1016/0014-5793(91)81385-l
Abstract
The requirement for a proline residue at the C‐terminus of the Ca2+‐binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C‐terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C‐terminal proline deleted showed a small change in secondary structure. In all cases studied, the C‐terminal proline was required for bioluminescence activity.Keywords
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