An in Vitro Demonstration of Cellular Sensitivity in Experimental Autoimmune Nephrosis in Rats

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Abstract
The migration of mono-nuclear cells from the spleens of rats with experimental autoimmune nephrosis was inhibited in tissue culture by extracts of rat kidney (p< 0.001) in both allogeneic and isogeneic strains. The migration was partially inhibited by the presence in the medium of rat liver (p = 0.01-0.02) but not by other tissue extracts. This effect appeared whether or not circulating precipitating antibody against kidney antigens could be demonstrated. The specificity of this reaction was further demonstrated in aminonucleoside nephrosis which involves no known immunologic mechanism; splenic monocytes from these animals, as well as from control animals, failed to show any inhibition of migration in the presence of these same antigens. There was no correlation between the degree of inhibition and the severity of disease. The role, if any, played by this specific cellular sensitivity in the pathogenesis of autoimmune nephrosis remains to be elucidated.