Indigenous Plasmids inPseudomonas solanacearum

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Abstract
Twenty-two of 39 strains of P. solanacearum harbor 1 or 2 plasmids with relative masses that range from 5.0 to > 500 megadaltons. When plasmid DNA from strains 85, 26 and 10 was digested with Bam H1 and Eco RI, very different fragment patterns were obtained, suggesting that each replicon represents a unique plasmid. The molecular weights of these plasmids, as calculated from the restriction patterns, are 84 .times. 106, 93 .times. 106 and 120 .+-. 106, respectively. One strain(mps5) harbored 2 small plasmids. A restriction enzyme map of the smaller (5-megadalton) plasmid was obtained. This plasmid, pW15, may be useful in the construction of a stable cloning vector for this species. There was no apparent relationship between the presence of plasmids and host of origin, natural resistance to antibiotics, or geographic origin of the strains examined.

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