^C-Nuclear Magnetic Resonance Spectra of Lacto-N-tetraose Derivative

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Abstract
The completely proton-decoupled 13C-NMR spectrum of the 1,6-anhydro derivative of O-.beta.-D-galactopyranosyl-(1 .fwdarw. 3)-O-2-acetamido-2-deoxy-.beta.-D-glucopyranosyl-(1 .fwdarw. 3)-O-.beta.-D-galactopyranosyl-(1 .fwdarw. 4)-D-glucopyranose (lacto-N-tetraose) (8) was measured in D2O. The signals were assigned by direct comparison of the spectra with those of 1,6-anhydro-.beta.-lactose (lactosan) and O-.beta.-D-galactopyranosyl-(1 .fwdarw. 3)-O-2-acetamido-2-deoxy-.beta.-D-glucopyranose (lacto-N-biose I), by examination of the selectively proton-decoupled and the partially relaxed Fourier transform (PRFT) spectra. The effect of the glycosylation shift was discussed, and the shift values calculated served for the confirmation of the structure of 8.

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