Effect of Porcine Growth Hormone-Releasing Factor on the Release and Synthesis of Growth Hormonein Vitro

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Abstract
Addition of small amounts of highly purified porcine growth hormone-releasing factor (GRF) to rat anterior pituitaries incubated in vitro for 6-24 hr. in Krebs-Ringer bicarbonate media significantly increased the release of growth hormone (GH). This effect was abolished by actinomycin D. Although more GH was released in vitro under the influence of GRF, the GH content of pituitaries from normal rats exposed to GRF was essentially unchanged after the incubation. When the pituitaries of donor rats were depleted of GH by a prior exposure of rats to cold in vivo, GRF was shown to stimulate the synthesis of GH in the incubated tissue as well as the release of GH. The results lend support to the thesis that one substance is responsible for both the release and synthesis of GH.