Cobalt-ion chelate affinity chromatography for the purification of brain neutral α-d-mannosidase and its separation from acid α-d-mannosidase
- 15 August 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 222 (1) , 261-264
- https://doi.org/10.1042/bj2220261
Abstract
A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single step with 100% recovery.This publication has 15 references indexed in Scilit:
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