Measurement of myometrial blood flow in rabbits by washout of Xenon-133 after atraumatic local labelling

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Abstract
In vivo experiments were performed on the uterine tissue of non-pregnant estradiol treated rabbit anesthetized with sodium pentobarbitone. The average blood flow calculated from the washout of Xenon-133 after atraumatic labelling from the uterine surface was 25.4 ml-min-1-(100 g)-1 (range 7.4–66.6), and after local injection of the tracer in isotonic saline directly into the myometrium 24.4 ml-min-1-(100 g)-1(range 8.7–45.3). During the experiments a monoexponential washout curve was found for the whole washout process both after atraumatic labelling and when the injected volume was 5μl. No trauma of injection was observed using this injection volume. The results support the applicability of a monocompartmental model for the washout of inert gas from the myometrium. Thus myometrial blood flow can be calculated from the Xenon-133 washout rate at any time interval during the washout process.