The oligosaccharides of human milk

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Abstract
The total fucose content of human milk samples was determined at intervals varying from 3 to 43 days post par turn. The amounts present remained fairly constant over this period and mean values of 128 mg/100 ml were obtained for each of 2 series, representing an oligosaccharide content of approximately 0.6 g/100 ml. The distribution pattern of fucose oligosaccharides absorbed on carbon-Celite columns and eluted with increasing strengths of ethanol was obtained for human milk samples. The oligosaccharides from human milk were purified by chromatographic methods; 5 of these are tentatively characterized for the first time as, di(lacto-N-tetraose), monofuco-di(lacto-N-tetraose), difuco-di(lacto-N-tetraose), monofuco-tri(lacto-N-tetraose), and difuco-tri(lacto-N-tetraose). A modification of the ethyl acetate-pyridine-water solvent, Jermyn, M. A. and Isherwood, F. A. (1949). Biochem. J. 44, 402, was developed which has special value for the separation of oligosaccharides more complex than a hexasaccharide.