High-Throughput Analysis of Hemoglobin from Neonates Using Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Open Access
- 1 June 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 48 (6) , 947-949
- https://doi.org/10.1093/clinchem/48.6.947
Abstract
The risks associated with sickle cell and other untreated hemoglobinopathies have led to mandatory neonatal screenings in 41 of the 50 states in the US (1). Preliminary screenings are often performed by electrophoresis at alkaline pH on cellulose acetate, with follow-up analysis of abnormal samples by acid electrophoresis on citrate agar (2). Alternatively, isoelectric focusing (IEF) or HPLC can be used in the screenings (3), but other techniques offer advantages.Keywords
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