Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome

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Abstract
In four cases of pulmonary toxoplasmosis occurring in patients with acquired immunodeficiency syndrome. Toxoplasma sp. was discovered in bronchoalveolar-lavage fluid (three cases) and in a lung biopsy specimen (one case) by using the following methods: direct examination of smears stained with eosine-methylene blue fast stain, indirect immunofluorescence assay, and inoculation of MRC5 fibroblast cell line in tissue culture.