Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP
- 7 January 2009
- journal article
- research article
- Published by International Union of Crystallography (IUCr) in Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Vol. 65 (2) , 102-104
- https://doi.org/10.1107/s1744309108042309
Abstract
Interleukin-22 (IL-22) is a pleiotropic cytokine that is involved in inflammatory responses. Human IL-22 was incubated with its soluble decoy receptor IL-22BP (IL-22 binding protein) and the IL-22-IL-22BP complex was crystallized in hanging drops using the vapour-diffusion method. Suitable crystals were obtained from polyethylene glycol solutions and diffraction data were collected to 2.75 Å resolution. The crystal belonged to the tetragonal space group P41, with unit-cell parameters a = b = 67.9, c = 172.5 Å, and contained two IL-22-IL-22BP complexes per asymmetric unit.Keywords
This publication has 26 references indexed in Scilit:
- Phasercrystallographic softwareJournal of Applied Crystallography, 2007
- Solvent content of protein crystalsPublished by Elsevier ,2006
- Structure of insect-cell-derived IL-22Acta Crystallographica Section D-Biological Crystallography, 2005
- IL-22 Increases the Innate Immunity of TissuesImmunity, 2004
- The expanded family of class II cytokines that share the IL-10 receptor-2 (IL-10R2) chainJournal of Leukocyte Biology, 2004
- Class II cytokine receptors and their ligands: Key antiviral and inflammatory modulatorsNature Reviews Immunology, 2003
- A novel, soluble homologue of the human IL-10 receptor with preferential expression in placentaGenes & Immunity, 2001
- A soluble class II cytokine receptor, IL-22RA2, is a naturally occurring IL-22 antagonistProceedings of the National Academy of Sciences, 2001
- Identification, Cloning, and Characterization of a Novel Soluble Receptor That Binds IL-22 and Neutralizes Its ActivityThe Journal of Immunology, 2001
- Identification of the Functional Interleukin-22 (IL-22) Receptor ComplexJournal of Biological Chemistry, 2001