Descriptions of Eubacterium timidum sp. nov., Eubacterium brachy sp. nov., and Eubacterium nodatum sp. nov. Isolated from Human Periodontitis

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Abstract
Three new species of Eubacterium, E. timidum, E. brachy, and E. nodatum, which were isolated principally from subgingival areas associated with periodontitis, are described. All are anaerobic, gram-positive, non-saccharolytic rods. They are differentiated from each other and from other non-saccharolytic species of eubacteria by acid and gas products in broth cultures and by morphological and growth characteristics. The type strains for the species are as follows: E. timidum ATCC 33093; E. brachy, ATCC 33089; and E. nodatum, ATCC 33099. These species were detected in 35 to 42% of the subgingival samples examined and, where present, constituted 3 to 57% of the cultivable flora. Strains of these species were isolated only occasionally from the adjacent supragingival areas sampled.

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