Boron Uptake in Mouse Brain Neoplasm

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Abstract
Experiments on the amount of B taken up by a brain tumor in mice, are described. Mice are injected with B containing compounds, killed and gross sections of the brain fixed by freeze-drying. Sections (approximately 5 u thick) containing tumor and normal brain are mounted on NTA plates and then irradiated with slow neutrons. Amounts of B in tissue are determined by measuring the a tracks in the emulsion from the B(n,a)Li reaction. Data indicate that approximately 30 times as much B/cm3 appears in the tumor edge when the mice are treated by injecting an Evans blue analogue dye, containing, B, as compared to the case in which borax is injected. Other B containing dyes which were used indicate corresponding ratios of about 15 and 10. From microscopic studies, it appears, qualitatively, that the dye is strongly taken up in the connective tissue, and especially in the growing edge of the tumor. Within the accuracy of the experiment, little or no dye appears in the normal mouse brain.