An improved method for calculating labeling indices of lung epithelial and interstitial cells
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Toxicology and Environmental Health
- Vol. 15 (2) , 229-236
- https://doi.org/10.1080/15287398509530649
Abstract
The sampling method was developed as an improvement in calculating labeling indices (LIs) of epithelial and interstitial cells in the airways and alveolar region of the lungs of rats. This method is more accurate and requires less time than the traditional cytokinetic methods, which involve counting all labeled and unlabeled cells. In the sampling method, all labeled cells are counted, but the total number of cells is estimated based on a representative sample count of airway and alveolar cells in each animal. The LIs as calculated by the 2 methods correlated very well (r > 0.97). The small amount of error introduced by estimating the total number of cells was more than compensated for by the increased accuracy associated with a larger sample size.This publication has 13 references indexed in Scilit:
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