COMPARISON OF GROWTH CHARACTERISTICS OF EXPERIMENTAL TUMOURS AND DERIVED CELL CULTURES

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Abstract
Cells from 7 rat tumors and a mouse sarcoma were transplanted in syngeneic animals and cultured in vitro. Tumors produced by inoculation of cultured cells in animals were compared with the primary tumors. For transplanted tumors, volume doubling times, Td, were compared with doubling times, Td(cult), of cell numbers in cultures. Volume doubling times of the transplanted tumors decreased with increasing volume. At volumes of about 0.5 cm3, Td values ranged from 2.2-10 days, while Td(cult) values ranged from 11-24 h. A systematic correlation between Td and Td(cult) could not be established. During sequential transplantation of the tumors for many generations, as well as during continuous propagation of derived cell cultures, significant changes occurred which resulted in a decrease in the expression of differentiation characteristics in tumors.