EFFECTS OF PROTEASE INHIBITORS ON CHEMICAL INDUCTION OF TYPE-C VIRUS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (8) , 2995-2999
Abstract
A role for proteolysis during chemical induction of endogenous xenotropic Type C virus from Kirsten sarcoma virus-transformed [K-BALB 19a] mouse cells was examined. Two distinct classes of protease inhibitors, the trypsin inhibitor .alpha.-N-tosyl-L-lysine chloromethyl ketone, and 2 naturally occurring oligopeptide inhibitors, antipain and leupeptin, inhibited induction of virus by cycloheximide and histidinol. Virus activation by 5-iododeoxyuridine was inhibited to a lesser degree. During the time cells were exposed to these compounds, there was little inhibition of [3H]uridine incorporation into total cellular RNA or polyA cytoplasmic messenger RNA, suggesting that inhibition of proteolysis and not RNA transcription, was responsible for blocking virus induction.

This publication has 0 references indexed in Scilit: