Phosphorylation of Chromatin-associated Proteins in Lemna and Hordeum
- 1 February 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 55 (2) , 288-292
- https://doi.org/10.1104/pp.55.2.288
Abstract
Sterile embryos of barley (Hordeum vulgare) and cultures of Lemna perpusilla have been labeled with 32Pi and the chromatin proteins prepared and separated by acid-urea and sodium dodecyl sulfate gel electrophoresis. Under these conditions chromatin proteins became labeled and the gel radioactivity profiles which were complex indicated a probable minimum of 15 to 20 proteins phosphorylated with molecular weights ranging from 104 to 105. The majority of the radioactivity, 80 to 90% of the total, is found in the acidic protein fraction and this can be recovered as serine phosphate after partial acid hydrolysis.Keywords
This publication has 11 references indexed in Scilit:
- Protein Kinase Activity Associated with Isolated Ribosomes from Peas and LemnaPlant Physiology, 1974
- The Phosphorylation of Ribosomal Protein in Lemna minorPlant Physiology, 1973
- Effect of in vitro histone phosphorylation on template activity of rat liver chromatinBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Description of two procedures which avoid the use of extreme pH conditions for the resolution of components isolated from chromatins prepared from pig cerebellar and pituitary nucleiBiochemistry, 1970
- Phosphorylation of ribosomal proteins in rabbit reticulocytes. Characterization and regulatory aspectsBiochemistry, 1970
- The Turnover of Nucleic Acids in Lemna minorPlant Physiology, 1970
- High resolution acrylamide gel electrophoresis of histonesArchives of Biochemistry and Biophysics, 1969
- Quantitative distribution of histone components in the pea plantBiochemistry, 1968
- Flowering Responses of the Long-day Plant Lemna gibba G3Plant Physiology, 1967
- HISTONE, A SUPPRESSOR OF CHROMOSOMAL RNA SYNTHESISProceedings of the National Academy of Sciences, 1962