EFFECT OF SPECIFIC GROWTH-RATE AND GLUCOSE CONCENTRATION ON GROWTH AND GLUCOSE-METABOLISM OF ESCHERICHIA-COLI K-12

  • 1 January 1976
    • journal article
    • research article
    • Vol. 17  (67) , 23-33
Abstract
Chemostat cultures of E. coli K-12 revealed that the metabolic change from respiration to aerobic fermentation can be obtained with increasing specific growth rate at low glucose input concentration (0.1%), or increasing glucose input concentrations at low specific growth rate (0.1 h-1). Both effects do not affect biomass formation. The metabolic change is not related to a pathway switch of glucose utilization. The increase in specific growth rate causes suppression of succinate dehydrogenase and NADH oxidase, but glucose increases cause suppression of succinate dehydrogenase, cytochrome a and 2-ketoglutarate dehyrogenase. Both phenomena are reflected in the specific O2 uptake rate, specific CO2 production rate and RQ values. Growth limitation could be related to a maximal glucose uptake rate of the cell and thus constitutes an entirely different effect caused by high glucose input concentration.

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