The fluorescence response of chlortetracycline‐loaded human neutrophils is modulated by prostaglandin E1, but not by cyclic nucleotides

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Abstract
Human neutrophils preloaded with chlortetracycline, commonly used as a probe of membrane‐bound calcium, demonstrate a prompt decrease in fluorescence when exposed to surface stimuli such as the chemotactic peptide fMet—Leu—Phe. The fluorescence response was highly sensitive to preincubation with prostaglandin E1. This effect was apparently not due to elevated levels of cAMP since exogenous dibutyryl‐cAMP did not alter the chlortetracycline fluorescence response to fMet—Leu—Phe. This is one of the few instances of prostaglandin E1 affecting neutrophils at physiologic concentrations, dissociated from changes in cellular cyclic nucleotide levels

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