The manometric estimation of nitrite in solution and in tissue

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Abstract
Manometric methods for the estimation of nitrite are based on the reaction of amidosulphonic acid with nitrite in the van Slyke or Warburg apparatus. The quantitative evolution of N2 from nitrite and NH2SO3H is not affected by the presence of NaCl, NaNO3, sucrose, glucose and muscle extracts. Nitrite in muscle can be estimated by the manometric analysis of a H2O extract of the tissue. With certain limitations nitrite in muscle can be estimated directly by the action of NH2SO3H on tissue contained in the Warburg apparatus. On mixing muscle and NaNOa there is both a rapid disappearance of a small amt. of nitrite (of the order of 10-5 g. NaNOs/ g. tissue) and a slow disappearance of nitrite.

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