Origins of conjugated triene fatty acids. The biosynthesis of calendic acid by Calendula officinalis

Abstract
Results from isotopic and incorporation experiments are consistent with formation of calendic acid form linoleic acid via a rearranged allyl radical, with loss of a hydrogen atom; since appropriate 9-hydroxy- and 9-hydroperoxy-diene acids were not converted into calendic acid by marigold seed homogenate, an elimination mechanism for formation of the 8(E)-double bond is not supported.
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