Enhanced recombination between λplac5 and mini-F-lac: The tra regulon is required for recombination enhancement

Publisher Website
Google Scholar
Abstract
F42lac recombination with λplac is normally 20-fold to 50-fold higher than recombination between λplac and a chromosomal lac gene. Transductional crosses with λplac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42lac.