CATHEPSIN B AND AMINOPEPTIDASE ACTIVITY IN THE POSTERIOR MIDGUT OF EUSCHISTUS EUSCHISTOIDES (HEMIPTERA: PENTATOMIDAE)
- 1 October 1984
- journal article
- Published by Cambridge University Press (CUP) in The Canadian Entomologist
- Vol. 116 (10) , 1393-1396
- https://doi.org/10.4039/ent1161393-10
Abstract
The posterior midgut of a seed-feeding pentatomid, Euschistus euschistoides (Vollenhoven), contains the proteinases cathepsin B and aminopeptidase. Cadiepsin B hydrolysis of benzoyl-DL-arginine-2-naphthylamide is activated by thiol chemicals and EDTA. Aminopeptidase hydrolysis of leucine-p-nitroanilide is activated by MgCl2 and inhibited by cysteine, glutathione, EDTA, and CaCl2. These results are similar to those obtained for cathepsin B and aminopeptidase from blood-feeding Hemiptera and support the hypothesis that catheptic proteinases are unique to this order.Keywords
This publication has 14 references indexed in Scilit:
- The preparation and properties of two new chromogenic substrates of trypsinPublished by Elsevier ,2004
- Cathepsin D-like activity in the posterior midgut of hemipteran insectsComparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1983
- Identification and partial characterization of digestive proteinases from two species of bedbug (Hemiptera: Cimicidae)Canadian Journal of Zoology, 1982
- Characterization of an acidic proteinase from the posterior midgut of Rhodnius prolixus stål (hemiptera: Reduviidae)Insect Biochemistry, 1982
- Identification and partial characterization of digestive proteinases from Triatoma phyllosoma pallidipennis stål (hemiptera: Reduviidae)Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1981
- Endoproteinase activity in the posterior midgut of Rhodnius prolixus Stål (Hemiptera: Reduviidae)Insect Biochemistry, 1980
- An improved color reagent for use in Barrett's assay of cathepsin BAnalytical Biochemistry, 1976
- Studies on digestive proteinase activity in the midgut of adult Dysdercus fasciatus (Hemiptera)Journal of Insect Physiology, 1972
- Digestion in InsectsPublished by Elsevier ,1970
- A simple colorimetric method for determination of proteinAnalytical Biochemistry, 1969