Liver‐colonizing melanoma cells selected from B‐16 melanoma

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Abstract
Liver‐colonizing tumor cells were selected from B‐16 melanoma by a stepwise procedure: (1) injection of the original cells through the portal circulation; (2) removal of tumor cells from the hepatic tumor nodules for culture in vitro; (3) reinjection of such cells through the portal circulation and repetition of steps 2 and 3 eight times. The predilection of these selected cells to form tumors in the liver was clearly demonstrated following injection of the cells intra‐arterially. The accuracy of the injection technique was controlled by monitoring amounts of injected radioactively labelled Sephadex microspheres. This liver‐colonizing preference was not exclusive, but relative.