Drosophila P-element transcripts are incorrectly processed in tobacco
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 11 (5) , 601-607
- https://doi.org/10.1007/bf00017460
Abstract
A gene encoding the Drosophila P-element transposase was expressed in transgenic tobacco plants under transcriptional control of the Drosophila HSP70 promoter. Polyadenylated transcripts were produced, but the major transcript was about 1 kb shorter than expected and the two introns were not removed. The 3′ end of the most abundant transcript occurred within the transposase coding sequence downstream of the acceptor site of the second intron within a region of high A+T content. These results provide an additional example in which RNA processing enzymes of plants utilize different mRNA processing signals than animals.Keywords
This publication has 32 references indexed in Scilit:
- Insertional Mutagenesis of the Drosophila Genome with Single P ElementsScience, 1988
- Splicing of plant pre-mRN As in animal systems and vice versaGene, 1987
- Plant cells do not properly recognize animal gene polyadenylation signalsPlant Molecular Biology, 1986
- Structural Analysis of Plant GenesAnnual Review of Plant Physiology, 1986
- The expression of a nopaline synthase ? human growth hormone chimaeric gene in transformed tobacco and sunflower callus tissuePlant Molecular Biology, 1986
- Identification and immunochemical analysis of biologically active Drosophila P element transposaseCell, 1986
- Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicingCell, 1986
- Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genomeCell, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 1983