Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.
- 1 August 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (15) , 5738-5742
- https://doi.org/10.1073/pnas.85.15.5738
Abstract
We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in .pi.H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of .alpha.-crystallin; the latter two may represent a response to stress.This publication has 34 references indexed in Scilit:
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