Ly-6A is required for T cell receptor expression and protein tyrosine kinase fyn activity.

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Abstract
To characterize the function of the Ly‐6A antigen in T cell activation, antisense Ly‐6 RNA was expressed in a stably transfected antigen‐specific T cell clone. Reduced Ly‐6A expression results in inhibition of responses to antigen, anti‐TCR (anti‐T cell receptor) crosslinking and concanavalin A plus recombinant interleukin 1 and causes impairment of in vitro fyn tyrosine kinase activity. More substantial reduction of Ly‐6A results in reduction of TCR expression. Analysis of mRNA species indicates that the reduction is specific for the TCR beta chain. These data demonstrate that Ly‐6A may regulate TCR expression and may be involved in early events of T cell activation via regulation of fyn tyrosine kinase activity.