SIMULTANEOUS DETERMINATION OF THE CONTENT OF SEROTONIN, DOPAMINE, NORADRENALINE AND ADRENALINE IN PANCREATIC ISLETS ISOLATED FROM FED AND STARVED MICE

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Abstract
A highly sensitive double isotope method for the simultaneous determination of serotonin, dopamine, noradrenaline and adrenaline was developed. Advantages and limitations of the method are discussed. The biogenic amines are all present in isolated pancreatic islet tissue from albino mice in concentrations ranging from approximately 5-30 .mu.mol per kg wet wt (0.8-5 .times. 10-3 pmol/ng DNA). A somewhat higher content of these amines, especially dopamine, was found in pancreatic acinar tissue. The hypothesis that the impaired glucose-induced insulin secretion during starvation partly is caused by an increased content of biogenic amines in the pancreatic islets was not supported by the experiments which showed an unchanged islet content of these amines after 48 h starvation.

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