Cloning, expression, characterisation and three-dimensional structure determination ofCaenorhabditis elegansspermidine synthase
Open Access
- 5 October 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (27) , 6037-6043
- https://doi.org/10.1016/j.febslet.2005.09.050
Abstract
The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S‐adenosylmethionine (K m = 110 μM) and a less pronounced feedback inhibition by the second reaction product 5’‐methylthioadenosine (IC50 = 430 μM). The C. elegans protein that carries a nematode‐specific insertion of 27 amino acids close to its N‐terminus was crystallized, leading to the first X‐ray structure of a dimeric eukaryotic SPDS.Keywords
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