The Development and Application of a Direct Radioimmunoassay for Plasma Aldosterone Using125I-Labeled Ligand—Comparison of Three Methods*†
- 1 January 1978
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 46 (1) , 105-113
- https://doi.org/10.1210/jcem-46-1-105
Abstract
A direct, rapid, sensitive, and highly specific radioimmunoassay for plasma aldosterone has been developed using aldosterone conjugated to [125I]histamine as ligand and a highly specific aldosterone antiserum. This assay permits the direct measurement of plasma aldosterone without preliminary extraction or purification steps and hence allows a single technician t o assay 500 samples in a week. An excellent correlation was obtained between the results of the direct assay and the levels measured after paper chromatography (n = 43,r = 0.99, P< 0.001) or after extraction (n = 43, r = 0.99, P <). The coefficients of variation for intra-assay and inter-assay determinations of samples from a normal plasma pool were 7.6% and 9.3% respectively. (J. Clin. Endocrinol. Metab. 46: 105, 1978.)Keywords
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