Immunoaffinity Column Coupled with Liquid Chromatography for Determination of Fumonisin B1 in Canned and Frozen Sweet Corn

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Abstract
A modified liquid chromatographic (LC) method for determining fumonisin B1 (FB1) in corn was applied to canned and frozen sweet corn. The corn is extracted with methanol–water (8 + 2), and the extract is filtered. The filtrate is diluted with water and passed through an immunoaffinity column. After the column is washed with water, FBi is eluted with methanol–water (8 + 2). The eluate is evaporated to dryness by using a vacuum concentrator, and the residue is dissolved in acetonitrile–water (1 +1). FB1 is derivatized with o-phthaldialdehyde. The derivative is separated on a reversed-phase C18 LC column using acetonitrile–water–acetic acid (50 + 50 + 1) and quantitated with a fluorescence detector. Recoveries of FB1 from canned and frozen corn spiked over the range of 50–200 ng/g were 76–88%. The limit of determination was about 25 ng/g, and the limit of detection was about 4 ng/g. The method was applied to 97 commercial canned and frozen sweet corn samples collected from different areas of the United States. Sixty samples contained no FB1. Low levels (trace–82 ng FB1/g corn) were found in 35 samples; 235 ng FB1/g was found in 1 canned corn sample, and 350 ng FB1/g was found in 1 frozen corn sample.

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