MARINE THIOBACILLI: I. ISOLATION AND DISTRIBUTION

Abstract
Bacterial colonies were isolated from Caribbean and Atlantic Ocean seawaters by the membrane filter technique and enrichment cultures in which thiosulfate was the only added source of energy. Although colonies were never abundant (0–275 per 100 ml), they have been recovered from the open ocean. The colonies were translucent to pale yellow on thiosulfate marine agar. The cells were Gram-negative motile rods (1–3 μ in length) requiring seawater for growth. The pH of the culture flasks dropped to 2.1 – 2.3 in some cases, and to 5.6 in others. On the basis of these observations, the colonies have been classified as marine thiobacilli.In seawater samples examined, the total reduced sulfur compounds ranged from 0 to 0.101 milliequivalents per liter.

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