Differential Cytokine Regulation of MHC Class II and Thyroglobin mRNAs in Rat Thyroid Cells

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Abstract
We have previously demonstrated that recombinant .gamma.-nerferon (.gamma.IF) inhibits thyroid cell proliferation in vitro. We now demonstrate differential regulation of thyroid cell genes by recombinant .gamma.IF, as evidence by data obtained measuring thyroglobulin (Tg) mRNA and MHC class II .alpha.-chain mRNA transcripts. Using the rat thyroid cellclone 1B-6, derived from FRTL-5 cells, .gamma.IF markedly increases MHC class II (TR1.D) .alpha.-chain transcripts in proliferating cells. Simultaneously, Tg mRNA transcript levels are markedly inhibited, even in the presence of TSH and insulin-like growth factor I (as insulin), known stimulators of Tg mRNA. Similar data were obtained for both FRTL-5 and FRTL parent cell lines. Furthermore, using probes to the 5'' region of the rat Tg gene we recognized not just a 9.0-kilobase (kb) mRNA, but also an additional 1.1-kb message in all proliferating thyroid cell cultures, suggesting truncation or alternative splicing of the Tg mRNA. Both the 9.0- and 1.1-kb mRNAs were inhibited by .gamma.IF. These data add further complexity to the cytokine regulation of thyroid cell gene expression and advise caution in making the assumption that thyroid cells may be efficient thyroid antigen-presenting cells in thyroid autoimmune disease.