QUANTITATIVE ADSORPTION OF CRYSTAL VIOLET

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Abstract
The uptake of crystal violet by bacterial cells can be measured quantitatively by micro-Kjeldahl analyses. As detd. by this method, heat-killed staphylococci adsorb more crystal violet than formalin-killed cells. Heat- and formalin-killed Salmonella typhosa cells adsorb the same quantity of crystal violet. 4 strains of Gram-positive organisms adsorbed 0.25 [plus or minus] 0.02 mg. of crystal violet N per mg. of bacterial N; 2 strains of Gram-negative organisms adsorbed 0.15 [plus or minus] 0.02 mg. of crystal violet N per mg. of bacterial N; 1 Gram-variable strain adsorbed 0.20 mg. [plus or minus] 0.02 mg. of crystal violet N per mg. of bacterial N.

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