Preparations of Rp‐cyclic adenosine 3',5'‐phosphorothioate (Rp‐cAMPS) can contain biologically active amounts of adenosine

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Abstract
Superoxide anion (O2 .−) production from human neutrophils stimulated by N-formyl-l-methionyl-l-leucyl-l-phenylalanine (fMLP, 1 μM) was inhibited by preparations of the inhibitor of cAMP-dependent protein kinase, Rp-cyclic adenosine 3',5'-phosphorothioate (Rp-cAMPS, 100 μM). This effect of Rp-cAMPS was reversed by xanthine amine congener (0.1 μM), an adenosine receptor antagonist, and by low concentrations of adenosine desaminase (0.02 mg/ml). HPLC analysis shows that these preparations of Rp-cAMPS contained concentrations of adenosine which could produce significant inhibition of fMLP-induced O2 .− production. These results suggest that Rp-cAMPS should be used with caution in cells or tissues containing adenosine receptors, and that preparations of Rp-cAMPS should be treated with adenosine desaminase before use to avoid activation of adenosine receptors.

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