Unscheduled DNA synthesis induced by 4-nitroquinoline-1-oxide in xeroderma pigmentosum cells and their complementing heterodikaryons
- 1 November 1980
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 6 (6) , 739-749
- https://doi.org/10.1007/bf01538973
Abstract
Unscheduled DNA synthesis (UDS) induced by 4-nitroquinoline-1-oxide (4NQO) in the complementation groups A to E and variant xeroderma pigmentosum (XP) cells, and various pairs of complementing XP heterokaryons were investigated. The pattern of UDS induced by various concentrations of 4NQO in normal cells was quite different from those in groups A to D XP cells. The patterns of UDS in group E and variant XP cells were indistinguishable from those of normal cells under our experimental conditions. The levels of UDS induced by 5×10−6M 4NQO were 13 % of normal in group A, 9% in group B, 17% in group C, 25 % in group D. The heterokaryons obtained by pair-wise fusions between different complementation groups of XP strains showed restored UDS induced by 5×10−6M 4NQO, while the dikaryons obtained from fusion between the same groups did not.Keywords
This publication has 22 references indexed in Scilit:
- Repair of DNA damage after exposure to 4-nitroquinoline-1-oxide in heterokaryons derived from xeroderma pigmentosum cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1980
- Excision repair of DNA base damage in human cells treated with the chemical carcinogen 4-nitroquinoline 1-oxideMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
- Complementing xeroderma pigmentosum fibroblasts restore biological activity to UV-damaged DNAMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
- The major cause of inactivation and mutation by 4-Nitroquinoline 1-Oxide in Escherichia coli: Excisable 4NQO-purine adductsJournal of Molecular Biology, 1975
- DNA Repair Monitored by an Enzymatic Assay in Multinucleate Xeroderma Pigmentosum Cells after FusionNature, 1974
- Repair replication in heterokaryons derived from different repair-deficient xeroderma pigmentosum strainsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- Studies on the substrate specificity of the T4 excision repair endonucleaseMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- High sensitivity of xeroderma pigmentosum cells to the carcinogen 4-nitroquinoline-1-oxideMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- Reduced DNA repair synthesis in xeroderma pigmentosum cells exposed to the oncogenic 4-nitroquinoline 1-oxide and 4-hydroxy-aminoquinoline 1-oxideMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1971
- ABSENCE OF EXCISION OF ULTRAVIOLET‐INDUCED CYCLOBUTANE DIMERS IN XERODERMA PIGMENTOSUM*Photochemistry and Photobiology, 1970