Rates of aminoacyl-transfer-ribonucleic acid synthesis in vivo and in vitro by bean leaves
- 1 May 1970
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 117 (5) , 853-859
- https://doi.org/10.1042/bj1170853
Abstract
1. A procedure for measuring rates of aminoacyl-tRNA synthesis in vitro and in intact leaves is presented. 2. Leaf discs showed rates close to those of intact leaves. 3. Cell-free preparations showed similar rates when assayed by pyrophosphate exchange, but actual aminoacyl-tRNA formation rates appeared to be much lower. Evidence is presented that dilution of supplied labelled amino acids was a major factor causing the low apparent rates. 4. Attempts to strip endogenous amino acids from plant tRNA resulted in low acceptor capability of the tRNA.Keywords
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