LIMITING DILUTION ANALYSIS OF ALLOANTIGEN-REACTIVE LYMPHOCYTES-T .4. ONTOGENY OF CYTOLYTIC LYMPHOCYTE-T PRECURSORS IN THE THYMUS
- 1 January 1981
- journal article
- research article
- Vol. 2 (4-5) , 245-255
Abstract
The ontogeny of development of cytolytic T lymphocyte (CTL-P) precursors in the murine thymus was investigated. Fetal or newborn thymocytes harvested from C57BL/6 mice were cultured with irradiated allogeneic (DBA/2) spleen cells in limiting dilution mixed leukocyte microcultures and assayed for cytotoxicity against 51Cr-labeled P-815 (DBA/2) tumor target cells. By applying Poisson statistics to the data, minimal thymic CTL-P frequency estimates were determined. CTL-P were barely detectable in fetal or newborn thymuses (3-19 CTL-P/106 cells), but their frequency increased dramatically (to 44-127/106 cells) within 24 h of birth. Control adult (day 70) thymuses contained 300-400 CTL-P/106 cells. Mixing experiments indicated that the low CTL-P frequencies in newborn thymuses was not due to a suppressive phenomenon. Strong indirect evidence for functional CTL-P maturation within the neonatal thymus is provided.This publication has 7 references indexed in Scilit:
- Cytolytic T lymphocyte precursors reactive against mutant Kb alloantigens are as frequent as those reactive against a whole foreign haplotype.The Journal of Immunology, 1980
- FREQUENCY OF ALLOREACTIVE CYTOTOXIC T CELL PRECURSORS IN THE MOUSE THYMUS AND SPLEEN DURING ONTOGENYTransplantation, 1979
- Ontogeny of Cell-Mediated Cytotoxicity: Induction of CTL in Early Postnatal ThymocytesThe Journal of Immunology, 1979
- LIMITING DILUTION ANALYSIS OF ALLOANTIGEN-REACTIVE LYMPHOCYTES-T .2. EFFECT OF CORTISONE AND CYCLOPHOSPHAMIDE ON CYTOLYTIC LYMPHOCYTES-T PRECURSOR FREQUENCIES IN THE THYMUS1979
- LIMITING DILUTION ANALYSIS OF ALLOANTIGEN-REACTIVE LYMPHOCYTES-T .1. COMPARISON OF PRECURSOR FREQUENCIES FOR PROLIFERATIVE AND CYTOLYTIC RESPONSES1979
- Ontogeny of Cell-Mediated Immunity of Murine Thymocytes and Spleen CellsDifferentiation, 1978
- QUANTITATIVE ASSAY OF LYTIC ACTION OF IMMUNE LYMPHOID CELLS ON 51CR-LABELLED ALLOGENEIC TARGET CELLS IN VITRO - INHIBITION BY ISOANTIBODY AND BY DRUGS1968