Human O6-alkylguanine-DNA alkyltransferase fails to repair O4-methylthymine and methyl phosphotriesters in DNA as efficiently as does the alkyltransferase from Escherichia coli
- 1 January 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 6 (7) , 949-953
- https://doi.org/10.1093/carcin/6.7.949
Abstract
The repair of alkylated poly(dT) annealed to poly(dA) and alkylated DNA was studied using human cells in culture, extracts of human liver and Escherichia coli, and partially purified O6-alkylguanine-DNA alkyltransferase from human liver. The human alkyltransferase behaved as if the transferring and accepting functions of the repair process resided on the same molecule. Extracts of E. coli efficiently repaired O6methylguanine, O4-methylthymine and methyl phosphotriesters in DNA. In contrast, cultured human cells and extracts from human liver repaired O6-methylguanine but did not repair O4-methylthymine or methyl phosphotriesters in DNA nearly as efficiently as did the E. coli extracts.This publication has 25 references indexed in Scilit:
- Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: Mutants deficient in thiols and mutants constitutive for the adaptive responseMolecular Genetics and Genomics, 1980
- A comprehensive quantitative analysis of methylated and ethylated DNA using high pressure liquid chromatographyCarcinogenesis: Integrative Cancer Research, 1980
- Formation by diethylnitrosamine and persistence of O4-ethylthymidine in rat liver DNA in vivoCancer Letters, 1980
- DNA synthesis with methylated poly(dC-dG) templates. Evidence for a competitive nature to miscoding byBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
- Repair of O6-methylguanine in adapted Escherichia coli.Proceedings of the National Academy of Sciences, 1978
- Reaction of DNA with alkylating agents. Differential alkylation of poly[dA-dT] by methylnitrosourea and ethylnitrosoureaBiochemistry, 1978
- Alkylation of deoxyribonucleic acid by carcinogens dimethyl sulphate, ethyl methanesulphonate, N-ethyl-N-nitrosourea and N-methyl-N-nitrosourea. Relative reactivity of the phosphodiester site thymidylyl(3′-5′)thymidineBiochemical Journal, 1978
- Preparation and template activities of polynucleotides containing O2- and O4-alkyluridine.Proceedings of the National Academy of Sciences, 1978
- The stability of methyl and ethyl phosphotriesters in DNA in vivoChemico-Biological Interactions, 1977
- The formation and stability of methyl phosphotriesters in the DNA of rat tissues after treatment with the carcinogen N,N-dimethylnitrosamineChemico-Biological Interactions, 1977