DEVELOPMENT AND DISTRIBUTION OF ACTINIDIN IN KIWIFRUIT (ACTINIDIA CHINENSIS) AND ITS PARTIAL CHARACTERIZATION
- 1 June 1988
- journal article
- research article
- Published by Hindawi Limited in Journal of Food Biochemistry
- Vol. 12 (2) , 109-116
- https://doi.org/10.1111/j.1745-4514.1988.tb00363.x
Abstract
The actinidin activity in kiwifruit increased 3-fold after 3 weeks postharvest ripening at 5°C with a 15% decrease in specific activity. Actinidin activity is highest in the pulp of ripe fruit at 27,600 U/kg fruit. The thiol protease actinidin from kiwifruit was extracted and purified 26-fold to a specific activity of 57 U/mg with Km= 91 μM and kcat= 101 s−1 towards N-α-CBZ-lysine p-nitrophenyl ester at pH 6.0 and 25°C.This publication has 9 references indexed in Scilit:
- Differences in the interaction of the catalytic groups of the active centres of actinidin and papain Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reactivity probesBiochemical Journal, 1981
- The specificity of actinidin and its relationship to the structure of the enzymeBiochimica et Biophysica Acta (BBA) - Enzymology, 1980
- The amino acid sequence of the tryptic peptides from actinidin, a proteolytic enzyme from the fruit of Actinidia chinensisBiochemical Journal, 1978
- The action of proteinase A2 of Actinidia chinensis on the B-chain of oxidized insulinBiochimica et Biophysica Acta (BBA) - Enzymology, 1973
- Kinetic studies on the thiol protease from Actinidia chinensisFEBS Letters, 1972
- The Kinetics and Mechanism of Papain-Catalyzed Hydrolyses1Journal of the American Chemical Society, 1966
- Kinetics of Papain-Catalyzed Hydrolysis of α-N-Benzoyl-L-arginine Ethyl Ester and α-N-Benzoyl-L-argininamide1Journal of the American Chemical Society, 1965
- Proteolytic enzyme of Actinidia chinensisBiochimica et Biophysica Acta, 1959
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951