Distinguishing between duplex and hairpin forms of RNA by 15N–1H heteronuclear NMR
- 27 June 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 347 (2-3) , 261-264
- https://doi.org/10.1016/0014-5793(94)00564-8
Abstract
A general method is described for distinguishing RNA hairpins from RNA duplexes by application of two‐dimensional filtered nuclear Overhauser enhancement spectra on a 1:1 mixture of unlabeled and 99% 15N‐labeled molecules. The method is applied to the RNA dodecamer rGGCGCUUGCGUC which can form an intramolecular hairpin under low salt conditions and a duplex in high salt. This procedure allows unambiguous identification of RNA hairpins or duplexes under the same conditions that are used in the NMR solution structure determination.Keywords
This publication has 16 references indexed in Scilit:
- Overcoming the ambiguity problem encountered in the analysis of nuclear Overhauser magnetic resonance spectra of symmetric dimer proteinsJournal of the American Chemical Society, 1993
- Novel DNA superstructures formed by telomere-like oligomersBiochemistry, 1992
- RNA structure and NMR spectroscopyQuarterly Reviews of Biophysics, 1991
- Structural Features that Give Rise to the Unusual Stability of RNA Hairpins Containing GNRA LoopsScience, 1991
- Solution structure of an unusually stable RNA hairpin, 5GGAC(UUCG)GUCCNature, 1990
- Heteronuclear filters in two-dimensional [1H, 1H]-NMR spectroscopy: combined use with isotope labelling for studies of macromolecular conformation and intermolecular interactionsQuarterly Reviews of Biophysics, 1990
- Nucleic acids and nuclear magnetic resonanceEuropean Journal of Biochemistry, 1988
- Calculating thermodynamic data for transitions of any molecularity from equilibrium melting curvesBiopolymers, 1987
- Exchangeable proton NMR without base-line distorsion, using new strong-pulse sequencesJournal of the American Chemical Society, 1982
- A two-dimensional nuclear overhauser experiment with pure absorption phase in four quadrantsJournal of Magnetic Resonance (1969), 1982