Histamine in the retina

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Abstract
Retina and choroid were rapidly and gently extracted from chickens, guinea‐pigs, rats, rabbits and cows. They were frozen, weighed and pooled in 3 % trichloroacetic acid in a refrigerator. Using a radioenzymic assay, histamine was found to be present in the retina of guinea‐pig (4.26 ± 0.677 nmol g‐1), rabbit (1.08 ± 0.422 nmol g‐1) and cow (0.189 ± 0.036 nmol g‐1), but not detected in chicken and rat. In the choroid, histamine was found in all species except rat (chicken: 4.92 ± 0.542 nmol g‐1; guinea‐pig: 121.5 ± 4.46 nmol g‐1; rabbit: 18.7 ± 3.93 nmol g‐1; cow: 0.36 ± 0.095 nmol g‐1).The study verifies the presence of histamine in the retina of some species, and thereby supports previous fluorometry assays.