Steroid Hormone Receptors in the Prostate
- 1 August 1979
- journal article
- research article
- Published by Wolters Kluwer Health in Journal of Urology
- Vol. 122 (2) , 170-175
- https://doi.org/10.1016/s0022-5347(17)56311-9
Abstract
Specific receptors for dihydrotestosterone and estradiol-17-.beta. were identified in cytosols of the human and baboon prostate. Binding of radioactive estradiol-17-beta to the 0.4 M KCl extractable component of human prostate nuclei also was demonstrated. Cyproterone acetate and diethylstilbestrol, agents of known high affinity for dihydrotestosterone and estradiol-17-beta receptors, respectively, did not bind significantly to sex hormone binding globulin and, therefore, were useful as competitors in distinguishing binding of dihydrotestosterone and estradiol-17-beta to sex hormone binding globulin and to their specific receptors. Displacement of [3H]-estradiol-17-beta binding by diethylstilbestrol in cytosols of 11 needle biopsy specimens (mean equals 16.8 mg.) from prostatic cancer patients was analyzed. These preliminary data indicated a trend towards greater competition by diethylstilbestrol for high affinity binding sites in differentiated tumor specimens from men who were not receiving estrogen therapy. Objective and subjective responses to hormone therapy were recorded in these patients, whereas the disease in those men with low displacement assay values progressed.This publication has 17 references indexed in Scilit:
- Characterization of the Binding of a Potent Synthetic Androgen, Methyltrienolone, to Human TissuesJournal of Clinical Investigation, 1978
- Characterization and Measurement of the Androgen Receptor in Human Benign Prostatic Hyperplasia and Prostatic CarcinomaJournal of Clinical Endocrinology & Metabolism, 1977
- Evaluation of the use of cyproterone acetate competition to distinguish between high-affinity binding of [3H]-dihydrotestosterone to human prostate cytosol receptors and to sex hormone-binding globulinThe Journal of Steroid Biochemistry and Molecular Biology, 1977
- Steroid receptors in the human prostate. Detection of tissue-specific androgen binding in prostate cancerClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Specific binding protein for 17β-estradiol in prostate with adenocarcinomaUrology, 1976
- A specific 17β-estradiol receptor in human benign hypertrophic prostrateBiochemical Medicine, 1976
- Evidence for a Specific Dihydrotestosterone-Binding Cytosol Receptor in the Human ProstateJournal of Clinical Endocrinology & Metabolism, 1975
- Steroid receptors in the human prostate: 1. Estradiol — 17β binding in benign prostatic hypertrophySteroids, 1975
- Androgen-Binding Proteins in Human Benign Prostatic HypertrophyJournal of Clinical Endocrinology & Metabolism, 1975
- Similarities between 5α-dihydrotestosterone-receptor complexes from human and rat prostatic tissue: Effects on RNA polymerase activityMolecular and Cellular Endocrinology, 1975